Legacy Columns
SIELC’s family of Legacy columns is based on the United States Pharmacopeia’s (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC’s Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
SIELC manufactures Legacy L1, Legacy L3, Legacy L7, Legacy L8, Legacy L9, Legacy L10, Legacy L11, Legacy L12, Legacy L13, Legacy L14, Legacy L16, Legacy L18, Legacy L20, Legacy L26, Legacy L28, Legacy L44, Legacy L78 and Legacy L85 columns.
As the HPLC world adapts to scientific advancements in separation technologies, old column technologies can easily become obsolete enough to warrant chromatography companies to stop producing these columns. Sometimes, companies will shut down only that production line, and other times, companies will shutter completely as they are unable to evolve quickly enough with the ever-changing landscape.
Consumers that once depended on these out-of-production columns are now stuck – they can either overhaul entire methods and systems, or they can abandon their projects altogether. With SIELC’s Legacy column series, consumers don’t have to do either.
SIELC’s Legacy column series is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. ple replacement. For example, L1 is one of the most popular designations, referring to columns with octadecyl silane (C18) chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter. An L1 column from one manufacturer can be swapped for another L1 column from a different manufacturer with little to no modification of the method. However, not all L1 columns are exactly the same, so relative compound retention and peak shape can be different from one brand’s column to another.
